Insertion junction: CLIP2.031425_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.031425
Chromosome:chromosome 2
Location:1708335
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g086000 ARS8 Arylsulfatase; (1 of 1) IPR002591//IPR003882//IPR017850 - Type I phosphodiesterase/nucleotide pyrophosphatase/phosphate transferase // Pistil-specific extensin-like protein // Alkaline-phosphatase-like, core domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTCCAGAAACTGAAACGCGCCGCCTGCACGCACGTTGCAAGGGGGATGT
Internal bar code:GTATATTTTCTTGCGGAGTGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1106
LEAP-Seq percent confirming:42.3077
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTGATGCCAAGTACATGC
Suggested primer 2:GTGCATGCATAGCTGTCAGC