| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.031457 |
| Chromosome: | chromosome 2 |
| Location: | 6814176 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388171 | (1 of 2) PTHR12905//PTHR12905:SF0 - SPERM PROTEIN HOMOLOG // METALLOPHOSPHOESTERASE DOMAIN-CONTAINING PROTEIN 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCAATACAACCGCATGCGTGCTTTGTGACTTTGTGTTGGTGGGGCGTC |
| Internal bar code: | AGTTACGGCTGGATGGGCTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 944 |
| LEAP-Seq percent confirming: | 86.1111 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCATGCCCTTACGCTTGC |
| Suggested primer 2: | GCCGTTCAAACTCAACCCAC |