Insertion junction: CLIP2.031466_3

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.031466
Chromosome:chromosome 12
Location:2898053
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g502700 (1 of 1) PF15665 - Family with sequence similarity 184, A and B (FAM184) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAACGGAATTTCATGGAATGACTGCACGGCATTTCGCGTGCGATTACTAG
Internal bar code:GAAGTCGCTTCTTTAGGTCAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1058
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:ACTTGTGTGGACCTGTGCAA
Suggested primer 2:ATGTGGGTCGTTGAGTGGAC