Insertion junction: CLIP2.031469_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.031469
Chromosome:chromosome 10
Location:619662
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g421800 (1 of 21) PF02469 - Fasciclin domain (Fasciclin) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTCCTGGATCCGGAGTTCTCTGGAACCGTGTTTGCGCCCGTGGATCTGG
Internal bar code:TGTTGCTTTCGAATCTGTGAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:3091
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:70
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:70

Suggested primers for confirmation by PCR

Suggested primer 1:ACACGCGTGTTGTTTGTAGC
Suggested primer 2:ATCTTGTCCCTCCCTCCCTC