Insertion junction: CLIP2.031493_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.031493
Chromosome:chromosome 17
Location:3710189
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g726000 PAP3 Class-II RNA nucleotidyl transferase 3, mitochondrial; (1 of 2) 2.7.7.72 - CCA tRNA nucleotidyltransferase / tRNA-nucleotidyltransferase intron
Cre17.g726050 Similar to Ubiquitin-Specific Protease; (1 of 1) K11843 - ubiquitin carboxyl-terminal hydrolase 14 [EC:3.4.19.12] (USP14, UBP6) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGGCTGGGACAGATCCACCTCAATGTCATTAAATGACTCTTTGCCCCA
Internal bar code:TGTTTGGTGTCTGAATTCATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2464
LEAP-Seq percent confirming:52.0833
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:AAGTTGGGGGTTTTGGCAGA
Suggested primer 2:CGCACCTTGACCTTCTCCTT