| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.031498 |
| Chromosome: | chromosome 16 |
| Location: | 5741581 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g680650 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGAAAGCACCTCGGACGCTGCTGGTCTCATGCCAGAGGCCGCCGTCAA |
| Internal bar code: | GAATGGAATCAGTTGACCACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3452 |
| LEAP-Seq percent confirming: | 96.1538 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTGCAGTGTACTGGTGA |
| Suggested primer 2: | CAGAAGCAGGAGAAGGGTGG |