Insertion junction: CLIP2.031571_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.031571
Chromosome:chromosome 9
Location:6098171
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g412880 HSP70F Heat shock protein 70F; (1 of 2) PTHR19375:SF173 - CHAPERONE PROTEIN DNAK CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCCGCCCCACCTGGACCCCAGGGACCCGCCGGCTGTAGCAGCCGCCGC
Internal bar code:TCGCACTCCACGGTAAACATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1058
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CAGACACCTGCGTTTGTTGG
Suggested primer 2:TGTACTGCTGGTGTTCGACC