Insertion junction: CLIP2.031598_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.031598
Chromosome:chromosome 1
Location:7973200
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g055600 KIL15 Putative kinesin-like motor protein; (1 of 2) K10400 - kinesin family member 15 (KIF15) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGACTTGGACGGCCTTCGGGATCGGCTCTTGGGCCCGCTCTCTCCCCCCG
Internal bar code:GATTCCGAGATGCAGTTTAGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:193
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GGACTACGTCTTCGCCAACA
Suggested primer 2:GCAGCGTGAATATGCAGTGG