Insertion junction: CLIP2.031630_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.031630
Chromosome:chromosome 8
Location:2144952
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g369800 DHS2 Deoxyhypusine synthase; (1 of 2) 2.5.1.46 - Deoxyhypusine synthase / Spermidine dehydrogenase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTCTCCGTGCAACAGGTGAGGAGCTGCGCAAGCAGGGCCTCAACCGCA
Internal bar code:CTATCGTCAGGTAATGGTCCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:5275
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:186
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:186

Suggested primers for confirmation by PCR

Suggested primer 1:GAACGCACACACAGTCACAC
Suggested primer 2:CAACCCGTGCTGTGAACTTG