Insertion junction: CLIP2.031645_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.031645
Chromosome:chromosome 1
Location:8130931
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g066552 TRXf1,TRXF1 Thioredoxin f1, chloroplastic; (1 of 7) 3.5.1.52 - Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase / N-oligosaccharide glycopeptidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCTGTCATGCTGTCAATCTGCTTGCTGTTGCGGTAGAGGTGGAAGGTG
Internal bar code:TTGGATATTATGTGTGAGTATC

Confirmation - LEAP-Seq

LEAP-Seq distance:1145
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GCTCAACCTGTCTCCTGCAT
Suggested primer 2:ATGCCCCAATTCTTCCCCTG