| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.031646 |
| Chromosome: | chromosome 10 |
| Location: | 4722439 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g452400 | CSF1 | Chlamydomonas specific family F protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCACAAACGCCGGCGCCCACTTCGAGCTACTGCCGTTACTGCTGCCAC |
| Internal bar code: | AGCGCTACTTAGTGGTGCTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5316 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 82 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 82 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGAATTTTGCCACGGACG |
| Suggested primer 2: | TGAACTGCTGCCTCCTTCTG |