| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.031652 |
| Chromosome: | chromosome 5 |
| Location: | 2033967 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g236500 | (1 of 1) 3.5.1.23 - Ceramidase / Acylsphingosine deacylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCTAGCTATCTGGCTGTGAGCCTGCTGATGCCTCCACACACAGACGGA |
| Internal bar code: | AAGCTACGGGGGCTCCCGGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5019 |
| LEAP-Seq percent confirming: | 98.2143 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCATACCCACGCTCTCTC |
| Suggested primer 2: | TGGGACTGTCTACGCACAAC |