| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.031660 |
| Chromosome: | chromosome 8 |
| Location: | 3249081 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g377150 | (1 of 1) 2.8.3.22 - Succinyl-CoA--L-malate CoA-transferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAATCGTTGCGCCCGGCGGTCTGCCGCGTAATCGACACTTCCCCTTCGTC |
| Internal bar code: | CCAATAGGACGTTAGTAGGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 557 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGAGGAAGGTGCGACCAG |
| Suggested primer 2: | CAAGACCAGCAAAGCCACAC |