Insertion junction: CLIP2.031668_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.031668
Chromosome:chromosome 11
Location:1442503
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g469500 FXL6 FixL-like PAS domain protein; (1 of 12) 2.7.13.3 - Histidine kinase / Protein kinase (histidine) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGACACAACCTTCGCGGGCGCCCACGCCGACCTGATGCGAGTGCTGCA
Internal bar code:AAGGTCTGGATGTTATTGCGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:3591
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:70
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:70

Suggested primers for confirmation by PCR

Suggested primer 1:TGCGAACTCACAGGAATGCT
Suggested primer 2:TCTTCTGCTGTGCCTTCTGG