Insertion junction: CLIP2.031687_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.031687
Chromosome:chromosome 2
Location:2237310
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g089900 SCM1,SCAMP5 (1 of 1) PTHR10687 - SECRETORY CARRIER-ASSOCIATED MEMBRANE PROTEIN SCAMP; secretory carrier membrane protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCCCTAGTCTAGTGCACGTGGTCGCATAACACTCATGCATGGCGCACG
Internal bar code:AGCGGCGGTGGTATCGCAAGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1346
LEAP-Seq percent confirming:94.7368
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:GGGCGTTTGATGCATAGCAG
Suggested primer 2:CATTGGATACTGCGCACGTG