Insertion junction: CLIP2.031694_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.031694
Chromosome:chromosome 10
Location:5874839
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g460600 CYN53 (1 of 2) PTHR11071//PTHR11071:SF297 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE // CYCLOPHILIN-LIKE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FAMILY PROTEIN; Cyclophilin 53 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACCAGCCCCTGCCTGCACGCCCCAATACTCATGCCTCCAAAGCTTCCAT
Internal bar code:CGTCCAACCATTTCCATAGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1110
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GGCGTAGGTACACACACACA
Suggested primer 2:GCACAAGGTAAGCATAGCGC