Insertion junction: CLIP2.031700_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.031700
Chromosome:chromosome 2
Location:7321410
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g391578 (1 of 4) 3.1.3.24 - Sucrose-phosphate phosphatase / Sucrose-6-phosphate phosphatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGAGTTGGCGCCTCCGCCTCCGCCCCCGCCTGCGCTGGCCGAAGCCCCA
Internal bar code:CAATGTAAGGTCTCACGCACGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2093
LEAP-Seq percent confirming:97.4359
LEAP-Seq n confirming:38
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:CTGGGAGTAGCATGGTAGCG
Suggested primer 2:ACAGTGACGTCGTCAAGGTC