Insertion junction: CLIP2.031732_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.031732
Chromosome:chromosome 16
Location:3129818
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g665050 CAV2 (1 of 1) IPR000048//IPR002077//IPR005821 - IQ motif, EF-hand binding site // Voltage-dependent calcium channel, alpha-1 subunit // Ion transport domain; Voltage-gated Ca2+ channel, alpha subunit intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGTAAATAGAGCAACACGTTACGCACCAGTTCTCCATGGTCAAAATTT
Internal bar code:GATCGTTTATAATCTAGCCTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1679
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GTTCATGTTCATCGCCTCGC
Suggested primer 2:CCCCGAATACCGACGAGAAG