Insertion junction: CLIP2.031753_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.031753
Chromosome:chromosome 8
Location:414048
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g358616 FAP398 Flagellar Associated Protein 398; (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGGTGGGGTGGTAGGACACGTAGCGGCTGAAGTCTGCGTCGCCGATGG
Internal bar code:TGGGGAACGTTACCTTCACACA

Confirmation - LEAP-Seq

LEAP-Seq distance:279
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:GTATGGGGTTGGGGTTCAGG
Suggested primer 2:AATCACACGCTTTCGACCCT