Insertion junction: CLIP2.031773_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.031773
Chromosome:chromosome 6
Location:6293687
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g292700 SNRK2G snRK1 family in Chlamydomonas, subgroup 2; (1 of 6) K14498 - serine/threonine-protein kinase SRK2 (SNRK2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTCCTTTTGCCTTCCCCCATTTGTCTGCCTCCATTATTCTCTATACGAC
Internal bar code:CTGCATGTCATCAGTTTCCCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1386
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AGACGTGTGCATACCTGGTG
Suggested primer 2:GAAGTTTGCAGAAGCCCAGC