Insertion junction: CLIP2.031777_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.031777
Chromosome:chromosome 12
Location:4410761
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g519950 FAP42,GUK3,C1b-350 Flagellar Associated Protein 42; (1 of 1) PTHR23359//PTHR23359:SF92 - NUCLEOTIDE KINASE // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTCCGGACGTGTTCGCCTACCCGCGCCACACCACTACCAGGCCCGCGC
Internal bar code:GACGCTAATCCGGCATCCAGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:3027
LEAP-Seq percent confirming:99.0291
LEAP-Seq n confirming:102
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:103

Suggested primers for confirmation by PCR

Suggested primer 1:CCACCACCTCGCAAAACAAG
Suggested primer 2:CGGCGACTACTTCTTTTGCG