Insertion junction: CLIP2.031779_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.031779
Chromosome:chromosome 10
Location:6532268
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g465450 (1 of 2) 2.8.2.20 - Protein-tyrosine sulfotransferase / Tyrosylprotein sulfotransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAGAGGCAGCGGCGGGGCAGCGGCGTCAGCTGCATCAGGCTCGGCTGGT
Internal bar code:CGCTAGAGTTAAATATGATAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:515
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CTTTTCCCCCATCCCTCACC
Suggested primer 2:GCGCAACACTCTTGCTGAAA