Insertion junction: CLIP2.031782_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.031782
Chromosome:chromosome 11
Location:663170
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467616 Dyf-13,IFT56,Ttc26 (1 of 3) PF12895 - Anaphase-promoting complex, cyclosome, subunit 3 (ANAPC3); Intraflagellar Transport Protein 56 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGTAGATGGTTGACTGGCGGCCTGTCCCGACGTCACAAAACCAGTCGC
Internal bar code:CTGGCTTATCTATACATAAGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:350
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGGGGGATTCAGGGGATTCA
Suggested primer 2:TGCAAGACTGGCAGAGACTG