Insertion junction: CLIP2.031809_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.031809
Chromosome:chromosome 1
Location:2775518
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g016950 (1 of 1) 2.5.1.58//2.5.1.59 - Protein farnesyltransferase / FTase // Protein geranylgeranyltransferase type I / Type I protein geranyl-geranyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGACGAGAAGAACTACCACGCCTGGGCGCACAGGCAGGCCGTCGTCAA
Internal bar code:GTGGCACGGCCGTGTGATAGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:561
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GCGGGTCAGAAGATGGTGAA
Suggested primer 2:CGGGCCGAAAGTAAAGGAGT