Insertion junction: CLIP2.031812_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.031812
Chromosome:chromosome 6
Location:2002110
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g265150 (1 of 1) K11833 - ubiquitin carboxyl-terminal hydrolase 2/21 [EC:3.4.19.12] (USP2_21) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCCGGACCGCCCCGGTCGCGTCACGCACAGGGGAAGAACCAAGTGCTGG
Internal bar code:GGGCATCCTTGGACGGGGATTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1383
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCTGCTGTTTACACGCTG
Suggested primer 2:GAAAACGGTTTGGGCTGTCC