| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.031816 |
| Chromosome: | chromosome 13 |
| Location: | 4495974 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g602950 | (1 of 1) IPR027050 - Nuclease SbcCD subunit C | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGTCATGTCAGCAGTGCGAGCCGCGCGCGGACCCCGTCGCCCAACTAG |
| Internal bar code: | TTTGTGGCTCATTCGGAGTATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 730 |
| LEAP-Seq percent confirming: | 45.4545 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAAGACGCAGAAGCAGAGA |
| Suggested primer 2: | GAGAAGGGTTGGGGTGGATG |