Insertion junction: CLIP2.031817_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.031817
Chromosome:chromosome 8
Location:2072821
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g369150 (1 of 1) PTHR10566:SF50 - PROTEIN LACT-3 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGCGGGAGCTGCGGAACAGCGGCGTGTGCGTGCAGCGAATCCCCGGGTG
Internal bar code:GTACGATCGAGTCCGACACGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:2308
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:ACTGTGCGGTGAGTCTGAAG
Suggested primer 2:ACCACCACACACACACACAT