Insertion junction: CLIP2.031838_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.031838
Chromosome:chromosome 12
Location:3078883
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g501150 MLT2 (1 of 31) IPR003590 - Leucine-rich repeat, ribonuclease inhibitor subtype; Multi eye protein 2 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGACTCTTCCACAATGAGCCGCAACACACAGCCGCGGTGCTCCAGGCGC
Internal bar code:AAGACATCTGAGTATCCTTGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1147
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:AGTAGGCGGTTGTTGGACAG
Suggested primer 2:AAGTAGAACCAGCGAGGCAC