Insertion junction: CLIP2.031843_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.031843
Chromosome:chromosome 16
Location:6353370
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g675700 BLZ21 (1 of 2) IPR000104//IPR004827 - Antifreeze protein, type I // Basic-leucine zipper domain; bZIP transcription factor CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGTTCAAGTCCGCAGCACTGTTCCCATGCGCCACATCAGCCGCGTTACC
Internal bar code:CGCATATTGTAGGTGAGTAGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1299
LEAP-Seq percent confirming:93.75
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:AGGGCGTAGTAGGTGCACTA
Suggested primer 2:GTAAGTCGTGTCAGGAGGCC