Insertion junction: CLIP2.031855_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.031855
Chromosome:chromosome 6
Location:408143
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g251650 PTC1 (1 of 1) K14430 - phosphate transporter (PHO87_91); Low-affinity phosphate transporter CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTGCGCGGCTTGCGGCCCACACGGGACAGGATGGACACCGCCACCTGGG
Internal bar code:GTAGCGGATCCATAGTGGACAT

Confirmation - LEAP-Seq

LEAP-Seq distance:671
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CTCACGCACACACACACTTG
Suggested primer 2:GAGGCATGTCCCAAGAACCA