| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.031862 |
| Chromosome: | chromosome 3 |
| Location: | 3844688 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g170200 | (1 of 2) PTHR10857:SF0 - PROTEIN GEM-4 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAATGGAACACCAGCCTGACCGAGGCGAATGGCACAGGAGACAAAGGGA |
| Internal bar code: | GAGGCCTCTTTTGTGTTAGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2162 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 75 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 90 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACGGAGATGATCGCAAAC |
| Suggested primer 2: | TTGGGTGGCCAAGTGTCTTT |