Insertion junction: CLIP2.031870_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.031870
Chromosome:chromosome 3
Location:8693363
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g800409 (1 of 5) IPR008930 - Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGGCAAGACTGGATGCTGTCGAAAAGGCGCGCGAGGCCCGATAATGAGC
Internal bar code:GTTTCTTAGTATAGATTAACAG

Confirmation - LEAP-Seq

LEAP-Seq distance:4080
LEAP-Seq percent confirming:84.7826
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCTGGCGCTTGACAATGA
Suggested primer 2:CCCAAAACTGCCTTGTTCGG