Insertion junction: CLIP2.031874_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.031874
Chromosome:chromosome 6
Location:6146897
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g291700 RSP3,PF14 (1 of 1) PF06098 - Radial spoke protein 3 (Radial_spoke_3); Radial spoke protein 3 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAGGGCTCCGGGGCAGTGTGCCCACTCACGTCGATGCCGGCGAGGGTCC
Internal bar code:TAGTATGCGTCTGCCTCTGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:6011
LEAP-Seq percent confirming:99.0991
LEAP-Seq n confirming:110
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:111

Suggested primers for confirmation by PCR

Suggested primer 1:GCTCGCCTGAGAGAGGATTC
Suggested primer 2:CGTTGCGAATCTGCTCGAAG