| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.031885 |
| Chromosome: | chromosome 3 |
| Location: | 4220714 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g173700 | KIL14 | Putative kinesin-like motor protein; (1 of 1) K10396 - kinesin family member 5 (KIF5) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGCCGCTGCCAGTCTGGCCGTACGCCATGATGGTGGCATTGATACCGC |
| Internal bar code: | CTTTGATTTTGGGAGCGTACAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1751 |
| LEAP-Seq percent confirming: | 98.6111 |
| LEAP-Seq n confirming: | 71 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTTCCAGCCTTTCTCCTC |
| Suggested primer 2: | CAGGCTGAACAGTTCCTCGT |