Insertion junction: CLIP2.031917_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.031917
Chromosome:chromosome 14
Location:183042
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g608800 NOL1,SDR24 (1 of 1) 1.1.1.294 - Chlorophyll(ide) b reductase / Chlorophyll b reductase; Chlorophyll b reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTACTTGCACTCCATTGCGCACGCCAATACACACACCTTTACACACCGTC
Internal bar code:GCAACTTGTTTTCGTGTGCTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:416
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CGGAAGCCTCCTTTCAACCT
Suggested primer 2:TGCCGAGGATTCAGCAGTTT