Insertion junction: CLIP2.031930_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.031930
Chromosome:chromosome 15
Location:849568
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre15.g640600 (1 of 1) PF00775 - Dioxygenase (Dioxygenase_C) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCCCCCAAGACACAGGGCCCCTCCCCCGGCTGCCGCCTCCCTGCCCCG
Internal bar code:ACGGGAGCAAATTACGGCAGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2480
LEAP-Seq percent confirming:86.6667
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:GCGAATACGACGACAACAGC
Suggested primer 2:CTAACCACCACCAACTGCCA