Insertion junction: CLIP2.031940_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.031940
Chromosome:chromosome 6
Location:2349564
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g267800 MML1,MITC8,MCP8 (1 of 1) K15119 - solute carrier family 25, member 39/40 (SLC25A39_40); MTM-Like Mn transporter 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAGATTGCTGTCCGCTGGAAGACGGGCGCTTGACCACATGCGCCACAA
Internal bar code:TTGTCGTATATGTCTAGAATTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1643
LEAP-Seq percent confirming:93.3333
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:CGACAACATCGCAAGCTAGC
Suggested primer 2:AGCTTCATCGACAGTGGTGG