Insertion junction: CLIP2.031940_3

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.031940
Chromosome:chromosome 14
Location:1950092
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAAAGTTGTTTCCGGCACTGTCGAAGTGGCGCGGTTGCATGAATGATTGC
Internal bar code:GAAGTAGTCGTCGTACATCGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1244
LEAP-Seq percent confirming:98.2143
LEAP-Seq n confirming:55
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:56

Suggested primers for confirmation by PCR

Suggested primer 1:ACATCACCCAGCTTGTAGGC
Suggested primer 2:ACACGCATCTGAGACTGCAA