Insertion junction: CLIP2.031945_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.031945
Chromosome:chromosome 9
Location:6041237
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g412600 CYG52 (1 of 1) PF00072//PF00211 - Response regulator receiver domain (Response_reg) // Adenylate and Guanylate cyclase catalytic domain (Guanylate_cyc); Adenylate/guanylate cyclase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCACAAATCAGCGCCGGCGCAGGCGCAGGCGCAGGCGGCGGCGCCGCCG
Internal bar code:ACCGCGAGGTCCCAGTTATAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1786
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:TAGTATCGCGAGCGTCACAC
Suggested primer 2:CGCACTCAACACCCTACACA