| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.031952 |
| Chromosome: | chromosome 12 |
| Location: | 2493526 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g507250 | (1 of 1) K18681 - DIS3-like exonuclease 1 (DIS3L) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGAATCGCTCGACGCCCTCGCGCAGCGAGCACAGCTGCTCGCTGCGCGG |
| Internal bar code: | AAAACACAGGCTTCCTTTTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 374 |
| LEAP-Seq percent confirming: | 82.9268 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTGCCTGTCTTGCCTAGC |
| Suggested primer 2: | GGCGCAATCGATCCTACTCA |