Insertion junction: CLIP2.031953_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.031953
Chromosome:chromosome 12
Location:747108
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g492600 FAP346,FAS3 FAS Domain Flagellar Associated Protein 346; (1 of 10) PTHR10900 - PERIOSTIN-RELATED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTGACGCTCTCTGTGGCTCTCCGCTTCACACGCTCAGGTCGTACGTGCA
Internal bar code:TTGTCTGGTGCGGGACTTTCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:3721
LEAP-Seq percent confirming:97.2973
LEAP-Seq n confirming:36
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:AACGAAACGAAAGCCAGTGC
Suggested primer 2:TACTCGGTCTACTCGGTGCA