Insertion junction: CLIP2.031972_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.031972
Chromosome:chromosome 11
Location:581436
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467607 (1 of 3) 2.7.11.17//2.7.11.25 - Calcium/calmodulin-dependent protein kinase / Microtubule-associated protein 2 kinase // Mitogen-activated protein kinase kinase kinase / MLTK CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCAGACCCAGGCTTGCGGCAGCCAGCTTCAGCGGTGGGTCCACCAGCAG
Internal bar code:TTAGGGGGATTACAGGGTCATC

Confirmation - LEAP-Seq

LEAP-Seq distance:4035
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:72
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:72

Suggested primers for confirmation by PCR

Suggested primer 1:TAACAAGGAGGAGCAGCTGC
Suggested primer 2:CACACAACGCCTGACTTTGG