| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.031973 |
| Chromosome: | chromosome 14 |
| Location: | 2871956 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g627150 | (1 of 1) PF05670//PF05833 - Domain of unknown function (DUF814) (DUF814) // Fibronectin-binding protein A N-terminus (FbpA) (FbpA) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCGTTAGTTGCAAGCGATAATACTTATGGGATGTGCGCCGAGGCGTCG |
| Internal bar code: | ACTATCATGGTGGTTTAATTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 775 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGCACGGGTGATAGGCAG |
| Suggested primer 2: | CAGATCTCACCCTGCGATCC |