| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.031981 |
| Chromosome: | chromosome 1 |
| Location: | 3316202 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g021400 | SPL13 | Pre-mRNA splicing factor; (1 of 1) PTHR16196//PTHR16196:SF0 - CELL CYCLE CONTROL PROTEIN CWF25 // PRE-MRNA-SPLICING FACTOR CWC25 HOMOLOG | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTAGAAGGACAAGGCAGGTGAGCGCAAGTTCCCAAACGCAGGAGTCAG |
| Internal bar code: | GGGATATAGATTAGTGCGACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4311 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGGAGTATGGCGGTGAG |
| Suggested primer 2: | AAGCTTTGCATGTTGCTGGG |