| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.031985 |
| Chromosome: | chromosome 13 |
| Location: | 460684 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g564950 | EFG13 | (1 of 1) K14536 - ribosome assembly protein 1 (RIA1); Ribosomal protein S5/Elongation Factor G/III/V family protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACAGGTTCCAAAGCACCGCCCCACCCCACCTGCAACAGCTGCTGCAGCC |
| Internal bar code: | TAAAGGTGGTGCAGACTTATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 889 |
| LEAP-Seq percent confirming: | 95.2381 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGTACACAGCAGCCTGAA |
| Suggested primer 2: | CGGTTTTGCTGGTGACCTTG |