Insertion cassette: | CIB2 |
Side of cassette: | 3' truncated? |
Strand: | - |
Strain: | CLIP2.031985 |
Chromosome: | chromosome 13 |
Location: | 460687 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre13.g564950 | EFG13 | (1 of 1) K14536 - ribosome assembly protein 1 (RIA1); Ribosomal protein S5/Elongation Factor G/III/V family protein | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTAAAGGCGGTTGGGTGCCGTTGTAAGCCGGGACGGCAACAGGCGCAA |
Internal bar code: | TAAAGGTGGTGCAGACTTATGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 610 |
LEAP-Seq percent confirming: | 86.6667 |
LEAP-Seq n confirming: | 13 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGGTTTTGCTGGTGACCTTG |
Suggested primer 2: | CGAGTACACAGCAGCCTGAA |