Insertion junction: CLIP2.031992_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.031992
Chromosome:chromosome 3
Location:4459031
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g175600 (1 of 1) PTHR26392//PTHR26392:SF78 - MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 7-RELATED // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGGAGCTGCCGTCGCACAAGCCGCCGGTGCCGCGGCGGTTGGAGACGGC
Internal bar code:GTGGTTATCTGTATACGCCTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:3345
LEAP-Seq percent confirming:93.75
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:TGACCTGCAGCATAAAGGCA
Suggested primer 2:GAATCACGTCAATGTCGCCG