| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.032002 |
| Chromosome: | chromosome 7 |
| Location: | 447976 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g315350 | (1 of 14) PF00566 - Rab-GTPase-TBC domain (RabGAP-TBC) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTTTGCGATCGCGATGGGGGAGCCAGTACGGCCAACCGCACATGCGGC |
| Internal bar code: | TGTCCGAAATCCTTTACTGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 974 |
| LEAP-Seq percent confirming: | 81.8182 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTCCACCTCCTTCTTGAA |
| Suggested primer 2: | TGTGCTCCGCTCGTTTTAGT |