Insertion junction: CLIP2.032003_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.032003
Chromosome:chromosome 13
Location:1982039
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g576600 Conserved Protein with Similarity to Catalytic domain of Protein Tyrosine Kinases; (1 of 1) PTHR23257//PTHR23257:SF405 - SERINE-THREONINE PROTEIN KINASE // MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 7-LIKE-RELATED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTGGTGCGGGTGCGCCGAGGCGGGCGCGTGCAGGCTGCCGGTGCCGCCG
Internal bar code:CTGATTTTCTATAAAGGAGAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1742
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:TAGGCGGCATGATGTCAACA
Suggested primer 2:CGCTCCAGCACTGCATAAAC