Insertion junction: CLIP2.032019_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.032019
Chromosome:chromosome 10
Location:4003561
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g447350 SEC23B (1 of 1) PF04810//PF04811 - Sec23/Sec24 zinc finger (zf-Sec23_Sec24) // Sec23/Sec24 trunk domain (Sec23_trunk); COP-II coat subunit intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGGGGGGTCGCTTTCTTGCCGCTTTGTTGGCGTTGTTCCTCACCACCA
Internal bar code:CGCACTGTAGCAGACATAACGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2573
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:GAGCACCTATACAGCGGGTC
Suggested primer 2:ACAACGGGGATGTCTGTGAC